At first, Nichols appeared in various television shows in bit parts (her first auditioned role, which she won, was that of an orgy-loving restaurant hostess in a 2002 episode of Sex and the City), but later that year she won the role of Jessica, the dogged school-newspaper reporter, in Dumb and Dumberer. She left Columbia midway through her last semester to shoot the picture, but still managed to graduate on time despite the demanding modeling schedule. She wrote two term papers and took the final exam of her undergraduate career just days before shipping all of her things to Atlanta, where Dumberer was being filmed.
Although Dumberer was a flop, Nichols earned roles in the television series Line of Fire, plus the 2005 horror movies, The Amityville Horror, and The Woods.
In 2004, FOX planned to develop a series vaguely reminiscent of their first hit drama, 21 Jump Street. They enlisted Todd & Glenn Kessler (of Robbery Homicide Division) to create the show, tentatively named The Inside. The Kesslers cast Nichols as a 22-year-old federal agent who impersonates a high-school girl in an undercover operation; they also cast Fastlane's Peter Facinelli and model Willa Holland, and shot a pilot. The pilot underwhelmed studio execs, though, and FOX brought in Angel writer Tim Minear to re-tool the concept. Minear ended up radically changing the show's story and purging the entire cast -- save for Nichols, who remained the show's centerpiece. While some sources said that Nichols was kept on because FOX pressured Minear to do so, Minear stood by a different story: "Even if [Nichols] wasn't already living in this show when I got there I'd have cast her. [She's] a star in the making, I feel. And an unspoiled delight..." he told Variety.
The new concept more closely echoed The Silence of the Lambs than Jump Street, and Nichols' character had been dramatically altered as well: now she was rookie Special Agent Rebecca Locke, assigned to Los Angeles' FBI Violent Crimes Unit, an elite group of criminal profilers charged with tracking the city's most dangerous deviants. Another of Minear's new wrinkles was that Nichols' character now had a marked similarity to the back-story of Elizabeth Smart, including a history of suffering, kidnapping, and abuse. The summer 2005 series received mixed reviews and a limited run, though the performances of Nichols (who says she "tested mostly for high school parts" before winning The Inside's dark lead role) and co-star Peter Coyote received generally favorable marks from critics.
After the failed FOX series, Nichols quickly found work on the ABC series Alias in the fall of 2005. Nichols portrayed Rachel Gibson, a computer expert duped into thinking she works for the CIA, when in fact she is working for a dangerous terrorist organization -- a predicament not far removed from that of Sydney Bristow in Alias' first season. Discovering the truth, Nichols' character later joins the real CIA and becomes Bristow's protégé, complete with undercover missions and martial arts scenes -- which Nichols had to work hard to make realistic, struggling at first with the stunts.[3] Coincidentally, Alias marked the second series in a row for Nichols in which she portrayed a government agent.
Although ABC announced the cancellation of Alias effective in May 2006, Nichols' character was created as a possible replacement for series star Jennifer Garner's Sydney, had the actress chosen to leave the show or scale back her involvement in the series (this, in fact, did begin to occur as the season progressed and Garner's real-life pregnancy prevented her from taking part in many action sequences). On May 22, 2006, Nichols appeared in Alias' final episode, "All the Time in the World".
After starring in two canceled television series in the last calendar year, Nichols is now turning her attention back to the big screen, with two movies set to come out in 2007. The first, Resurrecting the Champ, stars Josh Hartnett as a sportswriter who finds a former boxing legend (Samuel L. Jackson) living homeless on the streets. The second, P2, marks a return to the horror genre for Nichols, as she portrays a businesswoman who gets trapped inside a public parking garage with a deranged security guard. In this role, Nichols stood firm by her ethics and refused to shoot any type of nudity, including sheer, wet, tops. "In place of the nipples there's clearly a lot of cleavage," Nichols mused, "so we made a compromise."
Nichols also recently landed one of the leads in another FOX series -- the 2007 sci-fi drama Them, directed by Jonathan Mostow.
On November 7, 2007, Nichols confirmed that she would be cast in JJ Abrams' new Star Trek movie, but deferred commenting on which character she'd play.
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